Thursday, 21 September 2017

 

 Laboratory test and measures

 

The portable  lactate analyser (14.5 x 7.5 x 4.5, prototype II) powered with 4 rechargeable batteries (1.2 V each),has been calibrated (Figure 1) and validated with standard solution and applied “in field” by measuring the lactate variation during different training sessions (Figure 2). Disposable electrodes are commercial and modified in home.

 

Figure 1 shows the calibration curve obtained with the prototype II by analyzing standard solutions containing increasing concentration of lactic acid (0.1-300 mM).

This dynamic range covers the concentrations of lactate typically found in human sweat, urine and saliva at rest and during intense exercise. This range is by far more extended than that typical of commercial blood lactometer (typical range 0.2-22 mM).

Figure 1. Calibration curve obtained with the prototype II by analyzing standard solutions containing increasing concentration of lactic acid (0.1-300 mM range).

 

 

Figure 2. Results obtained using the Prototype II during two training sessions. Left y axis reports the watt of cicloergometer during training; right y axis shows the lactate values determined in sweat.

 

From Figure 2 it results that lactate variations clearly reflects the intensity of the exercise.

The prototype could be further improved and miniaturized.